Research Paper Volume 10, Issue 7 pp 1640—1648

Caloric restriction effects on liver mTOR signaling are time-of-day dependent

Figure 1. CR affects TORC1 activity across the day independently from BMAL1 and CRYs. Activity of mTORC1 in the liver of mice on AL and CR diets was assayed through phosphorylation of ribosomal protein S6 on Ser235/236. Representative WBs and quantification of diurnal rhythms in phosphorylation of S6 in wild type mice (upper panels), Bmal1-/- mice (middle panels) and Cry1,2-/- mice (lower panels). (AL) – black diamonds, black solid line, 30% caloric restriction (CR) – black squares and solid black lines. Two-way repeated ANOVA were used for statistical analysis, * - statistically significant difference (p<0.05) between diets (three male mice were used for every time point in both groups). Light and dark bars on the top of the figure represent light and dark phase of the day. ZT0 is the time when light is on and ZT12 is the time when light is off. Food for CR group was provided at ZT14. On the day of tissue collection at ZT14 tissues were harvested before mice received the food.