Figure 3. CR effect on AKT activity is time of the day and BMAL1 dependent. Activity of AKT in the liver of mice on AL and CR diets was assayed through phosphorylation of PRAS on S246. Representative WBs and quantification of diurnal rhythms in phosphorylation of PRAS in wild type mice (upper panels), Bmal1-/- mice (middle panels) and Cry1,2-/- mice (lower panels). (AL) – black diamonds, black solid line, 30% caloric restriction (CR) – black squares and dashed black lines. Two-way repeated ANOVA was used for statistical analysis, * - statistically significant difference (p<0.05) between diets (three male mice were used for every time point in both groups). Light and dark bars on the top of the figure represent light and dark phases of the day. ZT0 is the time when light is on and ZT12 is the time when light is off. Food for CR group was provided at ZT14. On the day of tissue collection at ZT14 tissues were harvested before mice receive the food.