Figure 4. Changes of mRNA and protein expression levels of LC3 in the spleen at 21 and 42 days of the experiment. (A) The relative mRNA expression levels. (B) The ratio of LC3II/LC3I protein expression. (C, D) The western blot assay. Data are presented with the mean + standard deviation (n=8), *p < 0.05, compared with the control group; **p < 0.01, compared with the control group.