Research Paper Volume 10, Issue 7 pp 1682—1697

Global quantitative TPA-based proteomics of mouse brain structures reveals significant alterations in expression of proteins involved in neuronal plasticity during aging

Figure 5. Immunofluorescent localization of selected glutamate ionotropic receptors which expression is altered by aging. Panels A-D show expression of AMPA receptor subunits: Gria1 (A and B) and Gria4 (C and D) in, respectively, hippocampus and cerebellum. Panels E-H show fluorescent signal associated with NMDA receptor subunits: Grin2a (E and F) and Grin2b (G and H) in, respectively, hippocampus and cortex. Panels A, C, E and G show the receptors localization in young, while the right panels (B, D, F and H) in aged animals. In each panel, the fluorescence related to studied proteins is shown in magenta (left images), the signal associated with MAP-2 protein is green (right upper image) and the merged image is presented in the left bottom image. CA3 – Cornu Ammonis area of hippocampus containing pyramidal neurons.