Research Paper Volume 10, Issue 7 pp 1698—1721

Implementation of environmental enrichment after middle age promotes healthy aging

Figure 10. Short-term EE modulates liver phenotypes in middle age mice. (A) Glucose tolerance test at 6-week in EE (n=10 per group). (B) Pyruvate tolerance test at 7-week in EE (n=10 per group). (C) Body and tissue weight at sacrifice after 8-week EE (n=10 per group). (D) Relative tissue mass calibrated to body weight (n=10 per group). (E) Liver triglycerides level (n=10 per group). (F) Liver glycogen level (n=10 per group). (G) Western blotting of livers. (H) Oil red O staining of livers. Scale bar, 50 µm. (I) Quantification of western blotting of the livers in (G), n=6 per group. * P<0.05, ** P<0.01, *** P<0.001. Values are means ± SEM.