Figure 8. Analyses of adipose tissues at the age of 22 months after 12-month EE. Gene expression profiles of (A) BAT (n=6 per group), (C) rWAT (n=6 per group), and (E) gWAT (n=6 per group). (B) Immunohistochemistry of BAT. (D) Immunohistochemistry of rWAT. (F) Immunohistochemistry of gWAT. Scale bar, 100 µm in H&E, 50 µm in UCP1 and PGC-1α staining. * P<0.05, ** P<0.01, *** P<0.001, + P=0.06. Values are means ± SEM.