Research Paper Volume 10, Issue 7 pp 1722—1744

Heme oxygenase-1 ameliorates oxidative stress-induced endothelial senescence via regulating endothelial nitric oxide synthase activation and coupling

Figure 5. HO-1 regulated endothelial senescence induced by H2O2 through HO-1-eNOS-Akt interaction. (A) Overexpression of HO-1 induced by Hemin enhanced the interaction between HO-1 and eNOS, and promoted the interaction between eNOS and Akt in senescent HUVECs induced by H2O2. *P < 0.05, Hemin+H2O2 vs. Hemin. n = 5. (B) Overexpression of HO-1 mediated by HO-1 recombinant adenovirus enhanced the interaction between HO-1 and eNOS, and promoted the interaction between eNOS and Akt in senescent HUVECs induced by H2O2. *P < 0.05, Ad-HO-1+H2O2 vs. Ad-HO-1. n = 5. (C) Overexpression of HO-1 enhanced the colocalization between HO-1 and eNOS, and promoted the colocalization between eNOS and Akt (400 × magnification) in senescent HUVECs induced by H2O2.