Figure 2. Levels of TNF-R1 in aging oocytes. Panels (A) and (B) are a merged image from laser confocal microscopy (original magnification ×400) showing localization of TNF-R1 in newly-ovulated oocytes. In the images, DNA and TNF-R1 were pseudo colored blue and red, respectively, and the bar is 10 µm. Image (B) is a negative control image showing an oocyte treated with the primary antibody against TNF-R1 omitted. Graph (C) shows levels of TNF-R1 (ratios of TNF-R1/GAPDH) by Western blot quantification in oocytes aging for different times in CZB medium. Each treatment was repeated 3 times with each replicate containing about 200 oocytes. Ratios of TNF-R1/GAPDH did not differ significantly (P>0.05) between culture times.