Figure 3. Ethanol activation rates and MPF activity after mouse DOs collected 13 h post hCG were cultured for 12 h in different media. To prepare CM, CCs were cultured in regular CZB medium for 24 (24hCM) or 36 h (36hCM) following a 24-h culture in CZB containing 200-µM H2O2. For controls (Ctrl), some newly ovulated oocytes were activated with ethanol or assayed for MPF activity immediately after recovery. For activation, each treatment was repeated 4-5 times and each replicate contained 25-30 DOs, and for MPF assay, each treatment was repeated 4-6 times with each replicate containing about 50 oocytes. a-d: Values with a different letter above bars differ significantly (P<0.05).