Figure 6. Concentrations of sTNF-α and sFasL in CM conditioned with CCs for different times. CCs recovered from newly ovulated oocytes were cultured in CZB medium containing 200-µM H2O2, and at different times of the culture, CM was recovered for ELISA measurement of sTNF-α and sFasL. One CM sample recovered was divided into two parts: one part for sTNF-α and the other part for sFasL measurement. Each treatment was repeated 3-4 times and each replicate contained 100 µl of CM from one culture well. Difference between sTNF-α and sFasL concentrations was insignificant (P>0.05) at all time points observed.