Research Paper Volume 10, Issue 11 pp 3089—3103

Glucose negatively affects Nrf2/SKN-1-mediated innate immunity in C. elegans

Figure 3. Glucose does not affect expression of marker genes of UPRmt or UPRER. (A) SKN-1 target gene (gst-4) but not marker genes of mitochondrial unfolded protein response (UPRmt) were affected by glucose. Animals raised on medium with and without glucose from L1 to L4/young adult stage were infected by S. typhimurium for 2 days. mRNA were extracted and reverse transcribed to cDNA. Quantitative RT-PCR was conducted using established primer sets (Table S4). P values were obtained by student’s t-test. **, P<0.001; ns, not significant. (B) Genes known to be induced by endoplasmic reticulum unfolded protein response (UPRER) were not affected by glucose. Animals raised on medium with and without glucose from L1 to L4/young adult stage were infected by S. typhimurium for 2 days. mRNA were extracted and reverse transcribed to cDNA. Quantitative RT-PCR was conducted using established primer sets (Table S4). Two independent experiments shows similar results and one of them are shown. P values were obtained by student’s t-test. ns, not significant. (C) Glucose and skn-1 RNAi knockdown is not additive in decreasing C. elegans’ lifespan. Lifespan and infection were carried out at 20 ºC. Animals raised on medium with and without glucose from L1 to L4/young adult stage were infected by S. typhimurium for 2 days, then transferred back to non-infected OP-50 bacteria plate for the rest of life. Survival were recorded every other day until all died. Data were collected from two independent experiments with number of worms >100. See Table S2 for details.