Figure 1. The expression of Tf and TfR was elevated in 3-month-old APP/PS1 Tg mice. The brains of 3-month-old APP/PS1 Tg mice were collected after anesthesia and were perfused (n=6). (A, C) The immunoreactivity of Tf and TfR was determined by immunohistochemistry with an anti-Tf and anti-TfR antibody, respectively. These images are representative of 6 independent mouse experiments, all of which produced similar results. (B, D) The protein expression of Tf and TfR was determined by western blot analysis. β-actin served as the internal control. Tf and TfR mRNA levels were determined by qRT-PCR, with the total amount of GAPDH serving as the internal control. The data represent the means±S.E. of all the experiments. **p<0.01; ***p<0.001 compared with WT controls.