Figure 2. Aβ plays a critical role in upregulating the expression of APH-1α/1β and PS1 in n2a cells and APP/PS1 Tg mice. (A) The brains of 3-month-old APP/PS1 Tg mice were collected after anesthesia and were perfused (n=12). (B) In select experiments, Aβ oligomers (500 ng/5 μl) or vehicle (PBS) was injected (i.c.v) into the ventricles of 3-month-old APP/PS1 Tg mice (n=10). (C) In separate experiments, n2a cells were treated with Aβ oligomers (1 μM) for 48 h. (A-C) The mRNA and protein levels of APH-1α/1β and PS1 were determined by qRT-PCR and western blots, respectively. The data represent the means±S.E. of all the experiments. *p<0.05; **p<0.01 compared with WT or vehicle-treated controls.