Research Paper Volume 10, Issue 11 pp 3117—3135

Transferrin is responsible for mediating the effects of iron ions on the regulation of anterior pharynx-defective-1α/β and Presenilin 1 expression via PGE2 and PGD2 at the early stage of Alzheimer’s Disease

Figure 5. Tf-TfR mediated the effects of Fe on the stimulation of the expression and metabolic activity of mPGES-1 and L-PGDS, which result in the synthesis of APH-1α/1β and PS1 in neurons. n2a cells were treated with Fe (10 μM) in the absence or presence of transfection with Tf or TfR siRNA. The mRNA and protein levels of mPGES-1, L-PGDS, APH-1α/1β and PS1 were determined by qRT-PCR and western blots, respectively. GAPDH and β-actin served as internal controls. The data represent the means±S.E. of all the experiments. **p<0.01; ***p<0.001 compared with vehicle-treated controls. ## p<0.01; ###p<0.001 with respect to Fe treatment alone.