Research Paper Volume 10, Issue 11 pp 3117—3135

Transferrin is responsible for mediating the effects of iron ions on the regulation of anterior pharynx-defective-1α/β and Presenilin 1 expression via PGE2 and PGD2 at the early stage of Alzheimer’s Disease

Figure 6. PGE2 and PGD2 antagonistically regulated the expression of APH-1α/1β and PS1 in n2a cells. (A, C) n2a cells were treated with Fe (10 μM) in the absence or presence of siRNA-targeted mPGES-1 or L-PGDS cDNA. (B, D) n2a cells were treated with PGE2 (10 μM) or PGD2 (1 μM) for 48 h. APH-1α/1β and PS1 were determined by qRT-PCR and western blots, respectively. GAPDH and β-actin served as internal controls. *p<0.05; **p<0.01 compared with vehicle-treated controls. # p<0.05 with respect to Fe-treatment alone.