Research Paper Volume 10, Issue 11 pp 3136—3147

Egr-1 is involved in coronary microembolization-induced myocardial injury via Bim/Beclin-1 pathway-mediated autophagy inhibition and apoptosis activation

Figure 7. The Egr-1/Bim/Beclin-1 pathway regulated autophagy and apoptosis during CME-induced myocardial injury. (A) Representative western blots of autophagy-associated proteins. (B) Representative western blots of apoptosis-associated and Egr-1/Bim/Beclin-1 pathway proteins. c-h Levels of the (C) LC3-II, (D) p62, (E) Beclin-1, (F) Egr-1, (G) Bim, and (H) cleaved caspase-3 normalized to GAPDH. The results are presented as the means ± SD from at least three independent experiments. aP < 0.05 compared with the Sham group; bP < 0.05 compared with the CME group; cP < 0.05 compared with the CME+shRNA group.