Research Paper Volume 10, Issue 11 pp 3148—3160

BK channel overexpression on plasma membrane of fibroblasts from Hutchinson-Gilford progeria syndrome

Figure 2. Immunofluorescence detection for BKCa channels expressed on plasma membrane in fixed cells. (A) Fluorescence micrographs of isolated hDF obtained from young and HGPS donors incubated with an anti-BKCa α subunit primary antibody visualized by FITC-conjugated secondary antibody and acquired at 200× magnification. Scale bars: 100 μm. (B). Quantification of mean fluorescence intensity of anti-BKCa antibody-stained cells. The green fluorescence intensity values are obtained from 30 cells (A.U. ± SEM). Significant differences calculated according to the Student’s t-test (p<0.05) are indicated.