Research Paper Volume 10, Issue 11 pp 3148—3160

BK channel overexpression on plasma membrane of fibroblasts from Hutchinson-Gilford progeria syndrome

Figure 5. Proliferation and adhesion rates without and with the BKCa inhibitor IbTx. (A) Average number of hDF obtained from young healthy donors and patients affected by HGPS estimated at 48, 72 and 96 hours from seeding and normalized at 24 h (fold-change ± SEM). Healthy and HGPS hDF were allowed to proliferate untreated (Young n=54; HGPS n=36) and treated by 100 nM IbTx (Young n=53; HGPS n=36). Young vs. HGPS: **p<0.001; ****p<5×10-7; Young vs. Young + IbTx: §p<0.01; HGPS vs. HGPS + IbTx: #p<0.01; ###p<0.0001. (B) Average percentage ± SEM of adherent hDF cells counted 2 h after seeding, treated (Young n=35, HGPS n=22) and untreated by 100 nM IbTx (Young n=40, HGPS n=24); image legend is the same as Figure A.