Research Paper Volume 10, Issue 11 pp 3229—3248

Figure 8. TG decreased tau hyperphosphorylation by decreasing the activity of tau related Ca²⁺-dependent kinases CDK5 and CaMKII. (A) Western blots of total Ca²⁺/calmodulin-dependent protein kinase II (CaMKII), phosphorylated CaMKII at Thr286 (CaMKII-pT286), total cyclin dependent kinase 5 (CDK5) and CDK5 activators (p35 and p25) in the hippocampal lysates. (B-F) Quantitative analysis of the blots, total CaMKII, CDK5, p35 and p25 were normalized to β-actin while CaMKII-pT286 was normalized to total CaMKII. (G) The ratio of p25/p35. Total protein levels of CaMKII and CDK5 were comparable among all groups, however their activities, as measured by increased CaMKII-pT286 for CaMKII and increase in both activators (p25 & p35) and ratio of p25/p35 for CDK5, were high in HHcy group and decrease to control level by TG treatment. The data were expressed as mean ± SEM (n = 6). # P < 0.05, ## P < 0.01, ### P < 0.001, #### P < 0.0001 versus control; ** P < 0.01, *** P < 0.001, **** P < 0.0001 versus HHcy.