Figure 4. SPC25+ PrC cells are enriched with CSCs. (A-B) The tumor sphere formation by SPC25- and SPC25+ cells from two cell lines, shown by representative images (A) and by quantification (B). (C-E) Docetaxel was applied to SPC25- and SPC25+ cells from both cell lines. (C) Cell viability by CCK-8 assay. (D-E) Cell apoptosis by TUNEL assay, shown by quantification (D), and by representative images (E). *p<0.05. N=5. Scale bars in A were 50 µm and in E were 20 µm.