Research Paper Volume 10, Issue 11 pp 3294—3307

Azithromycin and Roxithromycin define a new family of “senolytic” drugs that target senescent human fibroblasts

Figure 10. Azithromycin also shows senolytic activity in senescent BJ human skin fibroblasts. BJ cells were pretreated with BrdU for 8 days (to induce senescence), before they were exposed to Azithromycin for another 5 days. After that, SRB assay was performed to determine the effects of Azithromycin on cell viability. Azithromyin had a potent and selective effect on BJ cells, as it eliminated > 50% of senescent cells without reducing the viability of control cells after 5 days at 50 µM. These experiments were repeated at least 3 times independently, with very similar results. ** p<0.01.