Figure 7. Azithromycin strongly induces autophagy in MRC-5 cells. MRC-5 cells were treated with Azithromycin at 50 µM for 72 hours. Then, autophagy was monitored by detection of autophagic LC3 proteins with the Muse Autophagy LC3-antibody based kit. Azithromycin treatment resulted in more than a 3-fold elevation in autophagy in MRC-5 cells.