Research Paper Volume 10, Issue 11 pp 3294—3307

Azithromycin and Roxithromycin define a new family of “senolytic” drugs that target senescent human fibroblasts

Figure 9. Azithromycin has biphasic effects on oxygen consumption in MRC-5 cells. After 72 hours of treatment with Azithromycin (25 to 100 µM), MRC-5 cells were subjected to metabolic flux analysis with the Seahorse XFe96, which measures OCR (the oxygen consumption rate). Note that the highest dose (100 µM) triggered increased mitochondrial respiration, while the lower concentrations (50 µM) significantly reduced it. However, 25 µM did not have any significant effects on OCR. n=3; * p < 0.05.