Research Paper Volume 10, Issue 11 pp 3382—3396

Greater loss of mitochondrial function with ageing is associated with earlier onset of sarcopenia in C. elegans

Figure 2. Changes in lifespan did not change the order of progression of sub-cellular defects in C. elegans. (A) Temperature was used to lengthen (15°C) and shorten (25°C) lifespan in comparison to control (20°C) in animals with GFP-labelled mitochondria (median lifespan of 16d at 15°C vs. 10d at 20°C vs. 6d at 25°C, P<0.0001). (B) Mitochondrial fragmentation is greater at 25°C and lesser at 15°C across the lifespan. (C) Movement across the adult lifespan in GFP-labelled mitochondria is lower in 25C than other temperatures throughout the lifespan. (D) When data were expressed as % maximal lifespan the temporal progression of mitochondrial fragmentation was similar between strains. (E) When expressed as % maximal lifespan, the movement decline between 20°C and 25°C was similar to 50% of maximal lifespan. (F) Temperature was used to lengthen (15°C) and shorten (25°C) lifespan in comparison to control (20°C) in animals with GFP-labelled sarcomeres (median lifespan of 10d at 15°C vs. 8d at 20°C vs. 6d at 25°C, P<0.0001). (G) Decline in sarcomere structure was accelerated in animals at 25°C across the lifespan and sarcomere structure was preserved in 15°C compared to 20°C from 6d onwards. (H) Movement rates in animals with GFP-labelled sarcomeres were lower in animals at 25°C throughout the lifespan (I) Expressing data as % maximal lifespan did not appear to change the relationship between defect progression at different temperatures. (J) Movement expressed as % maximal lifespan shows that the rate of decline scales to lifespan. All data are presented as mean ± SEM. Scale bars in (B) and (G) represent 10 μm.