Research Paper Volume 10, Issue 11 pp 3382—3396

Greater loss of mitochondrial function with ageing is associated with earlier onset of sarcopenia in C. elegans

Figure 3. Protein degradation is accelerated in shorter-lived and delayed in longer-lived animals. (A) Temperature was used to lengthen (15°C) and shorten (25°C) lifespan in comparison to control (20°C) in animals with Lac-Z transgene (median lifespan of 22d at 15°C vs. 10d at 20°C vs. 6d at 25°C, P<0.0001). (B) The onset of increased protein degradation appears higher in 25°C animals and is delayed in 15°C animals. At 6d, insert shows that protein homeostasis is maintained at 15°C, somewhat maintained at 20°C and lost at 15°C. (C) Movement rates in animals with LacZ transgene show that movement decline is greater in 25°C animals and delayed in 15°C animals. (D) Rates of protein degradation expressed as % maximal lifespan shows that differences between temperatures are minimized. (E) Movement expressed as % maximal lifespan shows that until 50% maximal lifespan, differences between temperatures are minimized. Beyond 50%, movement rates are preserved in 20°C, but are lower throughout the remainder of the lifespan in 15°C in comparison to 25°C. Scale bar in B represents 80 μm.