Figure 5. Longevity at lower cultivation temperatures is associated with lower mitochondrial function and lower mitochondrial content. (A) At the onset of young adulthood, MAPR in worms with GFP-labelled mitochondria was significantly lower at 15°C, and greater at 25°C than at 20°C. GS denotes glutamate and succinate; GM denotes glutamate and malate; PyM denotes pyruvate and malate; PalM denotes palmitoyl-L-carnitine and malate; and S denotes succinate as respiratory substrate (n = 6, P<0.05 – P<0.001). (B) Maximal CS activity, indicative of mitochondrial content, was lowest at 16°C and highest at 25°C (n = 8, P<0.01). (C) Lipid peroxidation was not significantly different between animals at different temperatures (n = 6 – 11, P >0.05). Data represent mean ± SEM, * P<0.05; ** P<0.01; *** P<0.001.