Research Paper Volume 10, Issue 11 pp 3382—3396

Greater loss of mitochondrial function with ageing is associated with earlier onset of sarcopenia in C. elegans

Figure 6. Loss of mitochondrial function was an early event in ageing C. elegans. (A-E) At 2 d, the absolute difference between MAPR at 15°C, 20°C, and 25°C is greatly reduced versus 0d in animals with GFP-labelled mitochondria, with a range of respiratory substrates. At 2 d, 25°C was not significantly greater than 15°C or 20°C with any respiratory substrate (P >0.05). There was a significant reduction in MAPR over time (P<0.001), primarily at 0d – 2d with 25°C (all substrates P <0.05 – P <0.001) and 20°C (all substrates P<0.05 – P<0.001) but not 15°C (all substrates P >0.05). This same loss of MAPR was observed in wild-type C. elegans at 20°C (GS 0 vs. 2 d, P <0.05 and all substrates P< 0.001 0 vs. 4 d). Data represent mean ± SEM, * P <0.05; **P <0.01; ***P <0.001. (G) Assessment of mitochondrial membrane potential using JC-10 shows that mitochondrial membrane potential is preserved at 15°C (> 96h) whereas at 25°C, mitochondrial membrane potential is lost early in the lifespan (24-48h). Scale bar represents 25 μm.