Research Paper Volume 10, Issue 11 pp 3421—3437

miR-150-5p suppresses tumor progression by targeting VEGFA in colorectal cancer

Figure 2. miR-150-5p suppressed CRC cell proliferation, migration, invasion and HUVEC tube formation in vitro. (A) qRT-PCR was used to detect the expression of miR-150-5p in CRC cells transfected with antagomiR-150-5p or agomiR-150-5p and their negative control (antagomiR-NC, agomiR-NC). (B, C) CCK-8 (B) and colony formation (C) were performed to evaluate the proliferation abilities of CRC cells transfected with antagomiR-150-5p, agomiR-150-5p or negative control (antagomiR-NC, agomiR-NC). (D, E) Wound-healing (D) and transwell (E) assays were employed to detect the ability of migration and invasion of antagomiR-150-5p or agomiR-150-5p-transfected CRC cells and their negative control. (F) HUVECs were cultured in TCM derived from CRC cells transfected with antagomiR-150-5p or agomiR-150-5p and their negative control in 24-well Matrigel-coated plates. Each bar represents the mean ± SD of three independent experiments. *p<0.05, **p<0.01, ***p<0.001.