Research Paper Volume 10, Issue 11 pp 3421—3437

miR-150-5p suppresses tumor progression by targeting VEGFA in colorectal cancer

Figure 5. miR-150-5p inhibited CRC progression by targeting VEGFA. (A) VEGFA protein expression was determined in HCT116 and HCT8 cells transfected with agomiR-150-5p with VEGFA expression plasmid or empty vector using western blot; GAPDH was used as the internal control. (B-D) Cell proliferation (B, C), migration (D)and invasion (E) were evaluated in HCT116 and HCT8 cells transfected with agomiR-150-5p with VEGFA expression plasmid or empty vector. (F) HUVECs were cultured in TCM derived from HCT116 and HCT8 cells transfected with agomiR-150-5p plus VEGFA expression plasmid or empty vector. Data are shown as the mean±SD. *p<0.05, **p<0.01, ***p<0.001.