Figure 1. Increased senescence associated markers and TGF-β1 in the human corneal buttons CE with chronic graft rejection. (A) Clinical evaluation of corneal endothelial cells with chronic failure using alizarin red staining. (B-C) The mRNA expression of p16, p21 (B) and TGF-β1 (C) in the CE with or without chronic graft failure (**P<0.01,*P<0.05). (D-E) Representative photographs (D) and histopathology scores (E) for the IHC staining of p16 and TGF-β1 in the CE with chronic failure.