Figure 2. Metabolic profiles of granulosa cell (GC) media within or without ZEA-treatment. Utilizing the UPLC-QTOF detection method, total ion current diagrams vs. retention time of each group of GC media are shown (A), with Z1-Z10 mean 10 µM ZEA groups 1-10 and C1-C10 mean control groups 1-10 (0 µM ZEA). (B) Significantly different metabolites between the culture media with or without 10 µM ZEA treatment were marked in the cloud plot of mass to charge ratio (m/z) vs. retention time. Red point mean the ion of certain m/z showed decrease in ZEA groups compare with control groups. And green point in return, with the size of the point reflect the significance. Three-dimensional peak diagram of retention time, signal intensity, and m/z in blank control media (C), GC media without ZEA (D) or with ZEA treatment (E). (F) Principle component analysis (PCA) of the metabolic profiles among each sample between GC media without (red point) or with (cyan point) ZEA treatment.