Research Paper Volume 10, Issue 11 pp 3558—3573

Myoblasts rely on TAp63 to control basal mitochondria respiration

Figure 1. TAp63 knock-down affects the expression of genes involved in proliferation and metabolism. (A) RT-qPCR of p63 mRNA (Trp63) and (B) p21 (Cdkn1a) performed in proliferating Scr, sh1-p63 and sh2-p63 clones. Results are shown as average of three experiments ± s.d. *p<0.05. (C) Volcano plot showing -log10(FDR) in function of the log2(FC) for coding genes in Scr and sh1-p63. Green points indicate significantly expressed genes (D) GO terms of microarray performed on significantly modulated genes in C2C7 myoblasts (C). Panther was used for biological process (http://pantherdb.org/). (E) Heatmap expression level of genes from “positive regulation of cell proliferation” and “regulation of cellular metabolism” categories. (F) RT-qPCR analysis of mRNA level of modulated genes. Results are shown as average of three experiments ± s.d. *p<0.05, **p<0.01.