Research Paper Volume 10, Issue 11 pp 3558—3573

Myoblasts rely on TAp63 to control basal mitochondria respiration

Figure 2. TAp63 knock-down increases myoblast proliferation. (A) Growth curve of C2C7 Scr, sh1- and sh2-p63 clones. One representative experiment of three is shown. (B) EdU-incorporation assay in proliferating C2C7 scramble Scr, sh1- and sh2-p63 clones. Data are shown as mean± S.D. **p<0,01 by T-student test. (C) Clonogenicity assay of C2C7 Scr, sh1- and sh2-p63 clones. Images of one experiment of three are shown. Colony number count/dish is reported in the histogram (right). *p< 0,05 by T-student test. Data are shown as mean ± S.D. of three independent experiments. (D) Western blot confirming reduced expression of p21 and p57 in sh1- and sh2-p63. One representative experiment of three is shown. (E) Western blot analysis of C2C12 upon doxycyclin treatments (Tet-ON, Dox). Tubulin is shown as loading control. One representative experiment of three is shown. (F) Growth curve of Tet-ON TAp63γ C2C12 cells after 24, 48, 72 and 96h of Dox (2µg/ml) induction. (G) Clonogenicity assay of Tet-ON TAp63γ C2C12 cells after 6 days doxycycline (2µg/ml) induction. Colony number count/dish is reported in the histogram (right). Data are shown as mean ± S.D. of three independent experiments. *p< 0,05; **p< 0,01.