Research Paper Volume 11, Issue 1 pp 33—47

HPV-18 E2 protein downregulates antisense noncoding mitochondrial RNA-2, delaying replicative senescence of human keratinocytes

Figure 4. Changes in ncmtRNA levels in senescent HFK arrested at p8. The ncmtRNAs were amplified by RT-PCR from total RNA purified from HFK at p1, p3, p5 and p8, using 18S rRNA as an internal control. The experiments were run in triplicate and the relative band density was normalized to 18S for each case. (A) Representative gels showing RT-PCR amplification of these transcripts. (B) The relative expression of SncmtRNA was downregulated at p8. (C) In contrast, ASncmtRNA-2 increased progressively from p1 to senescence at p8 (***p<0.005). (D) Relative expression of ASncmtRNA-1 remained constant.