Figure 2. Calorie restriction protects against the loss of neurogenesis in the aged SVZ. (A) Cartoon showing the 3-dimensionally preserved SVZ wholemount. (B-E) Confocal projection images of immunohistochemistry for the neuroblast marker DCX performed on SVZ wholemounts in young and aged AL and CR mice. (F) Quantification of the amount of DCX immunostaining for the different groups. (G) Quantification of the number of BrdU+ cell in the olfactory bulb two weeks after BrdU injection. *** = p< 0.001, ** = p<0.01, two-way ANOVA, data are presented as mean ± SEM, n=5/group. Scale bar = 50 µm.