Figure 1. Analysis of the expression of the endogenous and the mutant CYLD protein in the K5-CYLDC/S mice. (A) Scheme of the construction used to obtain the K5-CYLDC/S mice. (B) Representative image showing the expression of K5 in the back skin of Control mice. Arrow: sebaceous gland; arrow head: ORS. (C) Analysis by WB of the expression of HA and CYLD in total protein extracts from the back skin of 30 day-old control and transgenic mice. Both lines of K5-CYLDC/S mice express higher levels of CYLD than Controls. HA was not detected in Control mice. GAPDH was used as a loading control. Immunostaining -with HA (D, F, H) and CYLD (E, G, I) antibodies- of back skin samples from Control (D, E) and transgenic (K5-CYLDC/S-X and K5-CYLDC/S-A) mice (F-G, H-I, respectively). HA is not expressed in Control mice (D). In the K5-CYLDC/S mice both, the expression of HA (F, H) and CYLD (G, I) follows the expression pattern of K5. Scale bars: 250μm (B); 150μm (D-I).