Research Paper Volume 11, Issue 1 pp 127—159

Premature aging and cancer development in transgenic mice lacking functional CYLD

Figure 12. The reduction of the NF-κB overactivation in keratinocytes expressing the CYLDC/S mutant decreases the expression of the biomarker of aging p16 and TNFα. HaCaT (Control and CYLDC/S) cells were treated with sodium salicylate for 48h when indicated (+). WB shows that HaCaT-CYLDC/S cells exhibit increased levels of expression of P-p65, p16 and TNFα, but the treatment with sodium salicylate, which reduced P-p65 levels, also decreases p16 and TNF-α levels in these cells. Actin was used as a control loading.