Research Paper Volume 11, Issue 1 pp 127—159

Premature aging and cancer development in transgenic mice lacking functional CYLD

Figure 2. Analysis of the hair regrowth in Control and K5-CYLDC/S mice. (A) Representative image of the patches of diffuse alopecia (arrows) of the transgenic mice (7-month-old mice are shown). (B, C) Representative images of the skin of 1-month-old mice. (D-L) Representative images of a hair growth experiment. The back of 7-week-old mice was shaved and the skin was collected 16 days after depilation. (D-F) Hair regrowth in Control mice. (G-L) Hair regrowth in the K5-CYLDC/S mice. (D, G, J) Representative images of freshly depilated mice of the corresponding phenotypes (day 0 of the experiment). (E, F) 16 days after shaving Control HFs were in the anagen-catagen I phase (E) and mice exhibited a homogeneous hair regrowth (F). (H) Histology showing initiation of the anagen phase of the hair cycle 16 days after shaving the back of a transgenic mouse. The macroscopic view of this mouse showed that hair was very short and hardly visible (I). (K) Histology of a section of the back skin of a K5-CYLDC/S mouse showing a delay in the growth of the new hair, so that 16 days after shaving it still remains in the telogen phase. (L) Macroscopically these areas correspond to those lacking hair in the back skin of the transgenic mouse (white asterisks). Red arrows show differences in the thickness of the skin between Control (E) and transgenic mice (H, K). Scale bars: 280 μm.