Research Paper Volume 11, Issue 2 pp 350—370

BCL2L10/BECN1 modulates hepatoma cells autophagy by regulating PI3K/AKT signaling pathway

Figure 4. BCL2L10 inhibited autophagy of Hep3B cells. (A)The expression of BCL2L10 in Hep3B cell line after transfection in three different groups was detected by qRT-PCR. (B) The cell viability was detected by CCK-8 in Hep3B cell line. The cell viability of si-BCL2L10 group was the weakest, while the cell viability of pcDNA3.1-BCL2L10 was the strongest. (C) The autophagy flux was observed under fluorescence microscope in Hep3B cell line. (D) The expression of LC3B-II/LC3B-I expression in the pcDNA3.1-BCL2L10 group was decreased, while the expression of LC3B-II/LC3B-I in the si-BCL2L10 group was increased. Besides, knockdown of BCL2L10 inhibited autophagic P62 degradation. (E) Accumulation of LC3B-II was also observed in the presence of Bafilomycin A1, while P62 expression was little decreased in Hep3B cells with BCL2L10 overexpression. * P<0.05, ** P<0.01.