Figure 7. Si-BCL2L10 induced autophagy of Hep3Bcells by releasing BECN1. (A) The expression of BCL2L10 and BECN1 after transfection in four different groups was detected by qRT-PCR. (B) The cell viability of si-BCL2L10 group was the weakest, while the cell viability of si-BECN1 group was the strongest. (C) Decrease of BECN1 could inhibit autophagic flux in HCC cells according to mRFP-GFP-LC3 assay. (D) The expression of LC3B-II/LC3B-I in si-BECN1 group was decreased while the expression of LC3B-II/LC3B-I in si-BCL2L10 group was increased. (E) Accumulation of LC3B-II was observed in the presence of Bafilomycin A1 in Hep3B cells. At the same time, knockout of BECN1 could not induced autophagic P62 degradation. * P <0.05.