Research Paper Volume 11, Issue 1 pp 230—239

Longer lifespan in the Rpd3 and Loco signaling results from the reduced catabolism in young age with noncoding RNA

Figure 1. Rpd3 and Loco regulate stress resistance and lifespan overlappingly. (A) The survival curve for oxidative stress using 2-day-old male flies. Control: UAS-loco/+; Rpd3-Down: rpd3- UAS-loco/+; Loco-Up: UAS-loco/actG4; Rpd3-Down+Loco-Up: rpd3- UAS-loco/actG4; p-value: log-rank test between the control and Rpd3-Down or Loco-Up. (B) Changes of oxidative stress resistance. The median survival times of flies under oxidative stress were calculated from several survival curves (A) and then the percentage change from control flies (0%) are represented as average ± standard error of mean (SEM) following normalization with the median of control flies (21.4 hours). P-value (*): Student’s t-test. (C) The lifespan of adult male flies. (D) Percent changes of mean lifespan are indicated as average ± SEM normalized by the control’s mean lifespan (UAS-loco/+: 39.4 days), which were calculated from several lifespan curves (C) of 2 ~ 4 independent experiments. The mean lifespan of another control +/actG4 flies was 40.1 days as single transgenic. (E) Venn diagram of differential genes with RNA-seq analyses between the control and Rpd3-Down or Loco-Up. Gene A and B indicate the groups changed with Rpd3-Down and Loco-Up, respectively. The C represents commonly changed genes in both Rpd3-Down and Loco-Up.