Figure 1. Rpd3 and Loco regulate stress resistance and lifespan overlappingly. (A) The survival curve for oxidative stress using 2-day-old male flies. Control: UAS-loco/+; Rpd3-Down: rpd3- UAS-loco/+; Loco-Up: UAS-loco/actG4; Rpd3-Down+Loco-Up: rpd3- UAS-loco/actG4; p-value: log-rank test between the control and Rpd3-Down or Loco-Up. (B) Changes of oxidative stress resistance. The median survival times of flies under oxidative stress were calculated from several survival curves (A) and then the percentage change from control flies (0%) are represented as average ± standard error of mean (SEM) following normalization with the median of control flies (21.4 hours). P-value (*): Student’s t-test. (C) The lifespan of adult male flies. (D) Percent changes of mean lifespan are indicated as average ± SEM normalized by the control’s mean lifespan (UAS-loco/+: 39.4 days), which were calculated from several lifespan curves (C) of 2 ~ 4 independent experiments. The mean lifespan of another control +/actG4 flies was 40.1 days as single transgenic. (E) Venn diagram of differential genes with RNA-seq analyses between the control and Rpd3-Down or Loco-Up. Gene A and B indicate the groups changed with Rpd3-Down and Loco-Up, respectively. The C represents commonly changed genes in both Rpd3-Down and Loco-Up.