Research Paper Volume 11, Issue 1 pp 230—239

Longer lifespan in the Rpd3 and Loco signaling results from the reduced catabolism in young age with noncoding RNA

Figure 2. Longer lifespan induced by the change of Rpd3 and Loco results from the reduced catabolism. (A) The gene expression analyses (Rpd3-Down or Loco-Up/control). From RNA-seq experiments using 2-day-old male flies, the genes changed more than 1.3-fold were selected with p-value <0.05 (Fisher’s exact test). The underlined genes represent that their expressions decrease (green) and increase (purple) in a different group, respectively. (B) With the genes oppositely changed in the Rpd3-Down and Loco-Up (*: total 39 in A), the gene ontology was analyzed using a DAVID web tool (http://david.abcc.ncifcrf.gov/home.jsp). %: involved genes/total 39 genes; p-value: a modified Fisher Exact. (C) Expressional changes of selected genes in RNA-seq and real-time PCR analyses. The fold changes were averaged from 4 ~ 13 independent experiments using four different RNA batches. Bold change: p-value < 0.05 ~ 0.0001. (D) Functional analyses (http://flybase.org) of genes which were selected with more than 1.5-fold changes and p-value <0.05 in both Rpd3-Down (longer lifespan) and Loco-Up (shorter lifespan) groups. *: genes tested as a representative of each functional subgroup with RT-PCR analysis in (C).