Research Paper Volume 11, Issue 1 pp 230—239

Longer lifespan in the Rpd3 and Loco signaling results from the reduced catabolism in young age with noncoding RNA

Figure 3. Noncoding RNAs related to the longevity of Rpd3 and Loco. (A) Expression analyses of the noncoding genes from RNA-seq experiments of Rpd3-Down or Loco-Up over the control. Among the genes with fold change > |1.3| and p-value < 0.05, the underline indicates that the expression decreases (green) and increases (purple) in each different group. (B) Expressional fold changes of selected genes with real-time PCR analyses (p-value < 0.02 ~ 0.0001). Loco-Dn: comparison between 2-day-old male flies of loco-/+ and wild-type (+/+); Old-Age: expression in the old flies (7 weeks) over young (1 week) flies between wild-type males; ns: non-specific change; *: expression changes of coding genes tested in Figure 2D. (C) The survival curve for starvation stress using 2-day-old male flies. P-value: log-rank test between the control (+/actG4) and CR45923/actG4 flies. (D-F) Stress resistance changes against starvation (D), heat (E), and oxidation (F). The percentages of median survival times changed from the +/actG4 flies (0%) are represented as average ± SEM followed by calculation of the median from several stress survival curves. P-value (*): Student’s t-test; I-rpd3: inverted sequence of rpd3. (G) The lifespan of adult male CR45923/actG4 flies with the controls (+/actG4 and CR45923/+). (H) Percent changes of mean lifespan are indicated as average ± SEM normalized by the +/actG4’s mean lifespan (39.3 ~ 42.8 days), which were calculated from several lifespan curves (G) of 3 ~ 5 independent experiments and were also tested with another driver tublin-Gal4. Parentheses: changed percentage out of data range in the graph.