Research Paper Volume 11, Issue 2 pp 448—466

miRNA-mRNA crosstalk in myocardial ischemia induced by calcified aortic valve stenosis

Figure 5. Biological processes and hub genes related to mitochondrial functions in CAVS. (A) Co-expression network approaches to reveal hub genes associated with mitochondrial functions in CAVS; (B) a. overview of hub genes and connected miRNAs. b. subnetwork of the integrated miRNAs and hub genes. The triangle and dot represent the hub genes and miRNA, respectively. Red color and up-direction represent up-regulated hub genes, blue color and down-direction represent down-regulated hub genes. The size color saturation of the nodes was weighted by the connectedness of the hub gene interacted with other miRNAs. c. two tail T-test of top-ten hub genes expression based on data profiles; C. Real-time PCR results of mRNA expression of these top-ten hub genes based on clinical samples; D. Western Blot results of protein expression of these top-ten hub genes based on clinical samples; *P<0.05, **P<0.01.