Research Paper Volume 11, Issue 2 pp 573—589

Figure 1. Characterization of rat fibroblast cultures. (A) The fibroblasts exit from tissue fragments reviewed under a microscope on the 4^th day in culture. (B) Typical spindle morphology of the fibroblasts evaluated after HE staining. All primary culture cells at the 25^th passage were confirmed to be positive for Vimentin by immunofluorescence microscopy (C), but occasionally some cells were positive for α-SMA as well (D).