Figure 1. Characterization of rat fibroblast cultures. (A) The fibroblasts exit from tissue fragments reviewed under a microscope on the 4th day in culture. (B) Typical spindle morphology of the fibroblasts evaluated after HE staining. All primary culture cells at the 25th passage were confirmed to be positive for Vimentin by immunofluorescence microscopy (C), but occasionally some cells were positive for α-SMA as well (D).