Research Paper Volume 11, Issue 3 pp 898—907

p38MAPK/SGK1 signaling regulates macrophage polarization in experimental autoimmune encephalomyelitis

Figure 2. p38MAPK/SGK1 signaling is required for IL-4-induced M2 macrophage polarization. (A) The levels of phosphorylation of p38 (p-p38), an active form of p38, were examined in macrophages with time after exposure to IL-4 by Western blotting. (B-F) Bone marrow derived macrophages were pretreated with vehicle (Vh; DMSO), or a specific inhibitor of p38MAPK, SB203580 (SB), or a specific inhibitor of SGK1, SI113 (SI), previous to IL-4 stimulation. (B-D) mRNA levels of Arg-1 (B), Ym-1 (C) and Fizz-1 (D) by RT-qPCR. (E) Arginase assay. (F) Flow cytometry for CD206 in Vh, SB or SI-treated macrophages that were exposed to IL-4. **p<0.01. *p<0.05. NS: non-significant. N=5.