Figure 3. Chitin-induced M2 macrophage polarization reduces the severity of EAE. C57BL/6 mice were immunized with MOG35-55 in CFA to induce EAE. Some of the MOG35-55-treated mice were randomly selected to receive intraspinal injection of Chitin. The other MOG35-55-treated mice received intraspinal injection of same amount of DMSO as controls. The development and severity of clinical signs in the two groups of mice (EAE or EAE+Chitin) were monitored longitudinally till day 21 after immunization, when the mice were sacrificed to evaluate the pathological changes in the spinal cord. (A) The clinical score. (B) The inflammation score. (C) The demyelination score. *p<0.05. N=10.