Research Paper Volume 11, Issue 3 pp 898—907

p38MAPK/SGK1 signaling regulates macrophage polarization in experimental autoimmune encephalomyelitis

Figure 5. Treatment with AAV-sh-p38 or AAV-sh-SGK1 attenuates the effects of Chitin on the severity of EAE. MOG35-55 was used to immunize the mice to generate EAE model, and in vivo macrophage polarization was induced by Chitin injection. AAV-sh-p38 or AAV-sh-SGK1 was given to the mice through intraspinal injection. The development and severity of clinical signs in 4 groups of mice were monitored longitudinally till day 21 after immunization, when the mice were sacrificed to evaluate the pathological changes in the spinal cord. (A) The clinical score. (B) The inflammation score. (C) The demyelination score. *p<0.05. NS: non-significant. N=10.