Research Paper Volume 11, Issue 2 pp 771—782

The curcumin analog EF24 is a novel senolytic agent

Figure 2. EF24 is a broad-spectrum senolytic agent. (A-D) Effect of EF24 on the viability of WI-38 non-senescent cells (NCs) (A), IR‐induced senescent cells (IR-SCs) (B), replication‐exhausted senescent cells (Rep‐SCs) (C), and Ras‐induced senescent cells (Ras‐SCs) (D) after the cells were treated with indicated concentrations of EF24 for 72 h. (E-H) Effect of EF24 on NCs, IR-SCs and Rep-SCs derived from IMR-90 fibroblasts (IMR-90) (E), human umbilical vein endothelial cells (HUVEC) (F), human renal epithelial cells (HREC) (G) and human pre-adipocytes (H) after they were treated with indicated concentrations of EF24 for 72 h. Data are represented as mean ± SEM of three independent assays. ***P < 0.001 and ****P < 0.0001 vs. vehicle control cells.