Figure 4. EF24 does not increase reactive oxygen species (ROS) production. (A) Representative flow cytometric plots of ROS assay. WI-38 NCs and IR‐SCs were treated with vehicle (Veh) or 2 µM EF24 for 3 h and 6 h. The levels of ROS in NCs and IR‐SCs were measured by flow cytometry after dihydrorhodamine 123 (DHR) staining. (B) and (C) ROS levels in NCs and IR-SCs after 3 h and 6 h treatment with 2 µM EF24, respectively, were presented as mean ± SEM (n = 3) of fluorescence intensity (MFI) of DHR.